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Optical imaging

Dernière mise à jour : lundi 29 août 2011, par Aline NONAT

Biomedical optical imaging is a rapidly growing field with various applications for disease diagnostic and molecular biology. Optical emissive probes can enable the acquisition of real-time bio-images with a remarkably high spatial resolution (fM-pM) and temporal resolution. A variety of different imaging and spectroscopic modes are available : light absorption, scattering microscopy, fluorescence and bioluminescence. [1] Fluorescence spectroscopy involves the presence of a fluorescent molecule (or probe) which is excited by an external source of illumination (lamp or LASER) and then emits photons at longer-wavelengths.

The majority of optical probes are based on organic fluorophores. [2] However, fluorescent nanocrystals made of semiconductor material (CdSe, CdTe,...), also called Quantum Dots (QDots), are emerging as a new class of fluorescent labels with improved properties (size-tuneable optical properties, high quantum yields, high extinction coefficients,…). [3]
Luminescent lanthanide complexes have also unique spectroscopic properties characterized by their long-lived and narrow-line emission. They are particularly interesting for the development of lanthanide-based time-resolved immunoassays, molecular probes for the quantification of pH, pO2 and various simple anions, DNA targeting and cell and tissue imaging. [4]

[1V. Ntziachristo, J. Ripoll, L. W. Wang, R. Weissleder, Nat. Biotechnol. 2005, 23, 313-320

[2H. Kobayashi, M. Ogawa, R. Alford, P. L. Choyke, Y. Urano, Chem. Rev. 2010, 110, 2620-2640

[3W. B. Cai, A. R. Hsu, Z.-B. Li, X. Chen, Nanoscale Res. Lett. 2007, 2(6), 265-281

[4S. V. Eliseeva, J.-C. G. Bünzli, Chem. Soc. Rev. 2010, 39, 189–227